A comparative study on the antioxidant activity of methanolic. In the previous chapters 3 and 4, it was shown that p. Leaf disc assays for rapid measurement of antioxidant activity. All plant extracts, except for tarragon, retarded peroxide formation at 80c the most effective being sage and sweet grass extracts fig. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to.
Researcharticle preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan. Commonly used medicinal plant extracts with standardized content of polyphenols were investigated for their total antioxidant activity taa. Phytochemical screening and antioxidant activity of ethanol extract. Antioxidant properties of plant extracts edelweiss publications. Antioxidant and potential antiinflammatory activity of. The present study reveals that extracts are a good source of antioxidant property containg phytoconstituents. Fruit extracts for total phenolics and antioxidant activity measured in methanol extract aoam analysis were prepared using the method of swain and hillis 1959, with some modi. Karthika abstract the aim of present study was to estimate the total phenolic and flavonoid contents in vitro antioxidant potential of methanolic leaf and root extracts of the herb. Screening of in vitro antioxidant activity of methanolic leaf. A comparative study on the antioxidant activity of. Among the extracts, tsb showed the highest antioxidant activity followed by trb, tf. The antioxidant activity of the two plant crude extracts against 2,2diphenyl1picrylhydrazyl dpph radical was determined by uv spectrophotometry at 518 nm. Preliminary phytochemical screening, quantitative analysis of alkaloids, and antioxidant activity of crude plant extracts from ephedra intermedia indigenous to balochistan rahmangul,1,2,3 syedumerjan,1,4 syedfaridullah,3 samiullahsherani,1 andnusratjahan3 1facultyofpharmacy,universityofbalochistan,quetta,pakistan. Evaluation of antioxidant activity of medicinal plant 885 figure 2.
The high contents of phenolic compounds indicated that these compounds contribute to the antioxidant activity. The effect of various plant extracts was measured on several important oxidation indicators. Antioxidant activity of some plant extracts of the family. If plant extracts contain antioxidant activity, it may clearup free radicals from the body, and boost the immune system to handle the infection or illness effectively. Inhibition of microsomal peroxidation data represents, mean sem, for n3 the reducing power of different commercial extracts was performed and showed. Antioxidant activity, active nitrogen species, sodium nitroprusside. Different concentrations of the plant extracts were prepared using analytical methanol 1, 3, 7, 10, 20, 30, 40. The present study was designed to evaluate the phytochemical screening, antimicrobial and antioxidant activities of anabasis aphylla l. Numerous works devoted to antioxidant activity of plant extracts are published. Antioxidant activity of polyphenolic plant extracts dimitrios stagos department of biochemistry and biotechnology, university of thessaly, 41500 biopolis, greece. Three grams of guava tissue were mixed with 25ml methanol and homogenized using the ultraturrax homogenizer. Screening of in vitro antioxidant activity of methanolic. There are many methods of evaluating the antioxidant activity of plant extracts, and in this study we have chosen to evaluate the antioxidant activity of plectranthus aromaticus extracts using two different methods.
Antioxidant activity, total phenolic and total flavonoid. Dpph free radical scavenging activity of the extracts of. Pdf different extracts from five medicinal plants in terms of phenolic compounds and antioxidant properties were studied. The antioxidant activities of methanol extracts of oregano, dittany, thyme, marjoram, spearmint, lavender and basil were tested in lard stored at 75c. Evaluation of antioxidant activity of medicinal plant. In the present study, three plant foods, namely, drumstick leaves moringa oleifera, mint leaves mentha spicata and carrot tuber daucus carota were extracted with ethanol and analyzed for their antioxidant activity. Antioxidant activity of polyphenolic plant extracts.
Antibacterial, antioxidant activity of ethanolic plant. Hence, in this study prepawe red polyphenol extracts from papaya peel pp with the aim of exploiting potential antioxidant and anticancer properties of pp extracts. Effect of extraction solventtechnique on the antioxidant activity of selected medicinal plant extracts article pdf available in molecules 146. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal plants rozina parul1, sukalayan kumar kundu 2 and pijush saha2 1. Screening of medicinal plant extracts for antioxidant activity.
A report on the in vitro antioxidant properties of vachellia karroo. Green tea, oligomeric procyanidins from grape seed and pine bark, bilberry, and ginkgo exhibited taa in the range of 5. Evaluation of antioxidant capacity of plant extracts by. Aug 19, 2015 the antioxidant activity of plant extracts is not limited to phenolics javanmardi et al. Antioxidant activity, total phenolic and total flavonoid contents of whole plant extracts torilis leptophylla l naima saeed, muhammad r khan and maria shabbir abstract background. The extracts were investigated for the antioxidant activity using 2, 2 diphenyl, 1 picryl hydrazyl. Preliminary phytochemical screening, quantitative analysis. The free radical scavenging action of methanol extracts of plant are in the order as desmodium gangeticum amaranthus caudatus solanum nigrum. The antioxidant activities of the methanol extracts from the leaves and stems of celtis africana ulmaceae were assessed in an effort to validate the medicinal potential of the subterranean part of the herb. Materials and methods fresh mature papaya fruits variety caribbean red were obtained from a local health food store, huntsville, al. Original article comparison of abts, dpph, frap, and orac. Evaluation of the in vitro and in vivo antioxidant.
Mar 16, 2001 screening of brazilian plant extracts for antioxidant activity by the use of dpph free radical method luciana l. In sum, the antioxidant activities of ethanolic extracts from m. The leaves extracts show a higher activity on cat and asa peroxidase activity, while the. To observe the antioxidant activity of leaves and seed extracts of n. The presence of flavanoids,tannins,steroids and saponins present in extracts may be responsible for antioxidant activity. Superoxide radical scavenging activity data represents, mean sem, for n3 figure 3. Jan 14, 2002 three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl. Phytochemical study and evaluation of the antioxidant. Antioxidant activity dpph free radical scavenging activity of methanolic extract the antioxidant activity of the plant extracts and the standard was assessed on the basis of the radical scavenging effect of the stable 1, 1diphenyl2picrylhydrazyl dpphfree radical activity by modified method.
The content of total phenolics in the extracts was determined spectrometrically according to the folin. Research article open access antioxidant activity, total. Consequently, the search and study of plant extracts that have the highest antiradical and antioxidant activity aoa is a very urgent task. Abstract identification and characterization of natural products with antioxidant and antiproliferative activity has received much interest over the past few years.
Antioxidant and apoptotic activity of papaya peel extracts in. Pdf methods for determining the antioxidant activity. The scavenging activity of the plant extract through the annihilation of the dpph radicals was investigated. From this study it is observed that all the plants posses marked antioxidant effect.
The present study evaluated the antioxidant effects of methanolic seed extract of a. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase. Antioxidant activity of some common medicinal plants. Pdf evaluation of antioxidant activity of some plant. Antioxidant properties of the methanol extracts of the leaves and stems of celtis africana. Antioxidant activity of various tea extracts in relation. Frap, dpph and total phenols by the folincitocalteau electron transfer methods and abts a method. Among the important biological properties exhibited by plant polyphenols, their antioxidant activity has raised a great interest 1. Antioxidant activity determination of citronellal and. Antioxidant activity of polyphenolic plant extracts mdpi.
Screening of brazilian plant extracts for antioxidant. Antioxidant properties of the methanol extracts of the leaves. Comparatively, methanolic extract showed higher activity than ethanolic activity. Total antioxidant activity and phenols and flavonoids content of several plant extracts claudiavalentina popa1, liliana lungu 2, manuela savoiu, corina bradu1, vasile dinoiu2, and andrei florin danet1 1faculty of chemistry, university of bucharest, bucharest, romania 2institute of organic chemistry c. Furthermore, it was estimated that more than 50% of prescribed drugs are either directly derived from herbsplants or active ingredients obtained from plant. In vitro antioxidant and antimicrobial activity of. For a typical assay an aliquot of 20 l of three dilutions of each extract in ethanol 0. The reacted solution obtained 1 ml was used for tba assay. Antioxidant properties of the methanol extracts of the. Felicia muricata is a medicinal plant used for the management of different human and livestock diseases in the eastern cape province of south. Pdf screening of medicinal plant extracts for antioxidant.
Extraction and determination of antioxidant activity of. Gc and carotene bleaching test bcbt, have been compared with regard to their application in the screening of plant extracts. Aframomum melegueta schum zingiberaceae is a perennial herb widely cultivated for its valuable seeds in the tropical region of africa. The antioxidative activity of a total of 92 phenolic extracts from edible and nonedible plant materials berries, fruits, vegetables, herbs, cereals, tree materials, plant sprouts, and seeds was examined by autoxidation of methyl linoleate. In these works aoa are given in units % mg, mgml or mgg, that character. Preliminary phytochemical screening, quantitative analysis of. Screening of plant extracts for antioxidant activity. Antioxidant activity determination of citronellal and crude. Antioxidant activity in extracts from coriander sciencedirect. Witch hazel, propolis epid, artichoke, and hawthorn afforded. A detailed study was performed on the antioxidant activity of the methanol extract of whole.
Evaluation of antioxidant activity of some plant extracts and their application in biscuits. Pdf in vitro antioxidant and antiproliferative activity. Evaluation of antioxidant activity of some plant extracts and. Dpph free radical scavenging activity of the extracts of the. The antioxidant activity of the plant extracts was examined on the basis of the scavenging effect on the stable dpph free radical activity braca et al. Phytochemical screening and antioxidant activity of moroccan. Antioxidant activity of methanol extracts of different parts of lantana. Ethiopia is one of the developing countries which have enormous diversity of plants.
Screening of plant extracts for antioxidant activity wiley online. Four complementary methods were used to determine the antioxidant activity of methanolic extracts. In vitro nitric oxide scavenging activity of methanol extracts of three bangladeshi medicinal. Pdf evaluation of antioxidant activity of medicinal plant extracts.
Phytochemical screening revealed the presence of alkaloid, flavonoid, saponin, terpenoid, steroid and sterols in the extracts of aerial parts of. The ethanolic extracts exhibited moderate antimicrobial activities, whereas the aqueous extracts showed poor antimicrobial properties in. Three methods widely employed in the evaluation of antioxidant activity, namely 2,2diphenyl1 picrylhydrazyl dpph radical scavenging method, static. Preliminary phytochemical screening, quantitative estimation.
Pdf in vitro antioxidant and antiproliferative activity of. Ferric reducing antioxidant potential assay frap assay the frap assay was employed to estimate the antioxidant capacity of the samples in vitro. Evaluation of the in vitro and in vivo antioxidant potentials. Therefore, plant oils are widely used as a substrate for testing antioxidant activity. Screening of in vitro antioxidant activity of methanolic leaf and root extracts of hypochaeris radicata l. The activity was measured according to the method previously described. The antioxidant activity of extracts was evaluated according to the amount of malonaldehyde mda formed by the feso 4induced oxidation of linoleic acid and a high pufa oil. However, the total antioxidant activities of plant extracts cannot be evaluated by using one single method, due to the complex composition of phytochemicals as well as of oxidative processes. Pdf antioxidant activity of polyphenolic plant extracts. Evaluation of antioxidant activity of some plant extracts.
Methanolic extracts of cassia fistula showed the highest amount of phenolic and flavonoid content and reducing capacity, whereas hexane extracts exhibited the lowest level of reducing capacity. Extract, free radical, antioxidant activity, medicinal plant. Preparation of plant extracts for antioxidant evaluation about 10 g of the grounded plant were soaked in 1 liter of methanol 99% and put in a shaker device at 100 rounds per minute for 72 hours at room temperature and stored in refrigerator for 4 days. Antioxidant activity % control dr dr sample dr control 100. The antioxidant activity may also contribute from the other antioxidant secondary metabolites such as volatile oils, carotenoids and vitamins wongsa et al.
Antioxidant activity of polyphenolic plant extracts by dimitrios stagos department of biochemistry and biotechnology, university of thessaly, 41500 biopolis, greece. Oregano extract was found to be the most effective in stabilizing lard, followed by thyme, dittany, marjoram and lavender extracts, in a decreasing order. Department of pharmacy, gono bishwabidyalay, savar, dhaka 44, bangladesh. The results indicated that both leaves and seed extracts have an antioxidant activity.
Comparative analysis of the antioxidant activity of cassia. Screening of antioxidant activity and phenolic content of. The antioxidant activity and phenolic contents of the stem as determined by the dpph, proanthocyanidins, total. Herbal plants considered as good antioxidant since ancient times. In vitro antioxidant activity of extracts from the leaves of felicia. Antioxidant, anticancer activity and phytochemical. Figure 1 shows the amount of each extract required for 50% inhibition of dpph activity ic 50. Antioxidant properties of several medicinal plants growing. The antioxidant activities were determined by total antioxidant capacity, dpph 1,1diphenyl2picrylhydrazine radical scavenging assay, hydroxyl radical scavenging assay, ferrous reducing antioxidant capacity and lipid peroxidation inhibition assay methods. Antioxidant activity of various tea extracts in relation to their antimutagenicity gowchin yen, and huiyin chen j. Antimicrobial and antioxidant activity of essential oil. Antioxidant and antilipid peroxidation activities of leaves. Phytochemical screening and evaluation of antioxidant and. The significant linear correlation was confirmed between the values for the total phenolic content and antioxidant activity of plant extracts.
In the present paper we have investigated antioxidant activity of extracts from withania somnifera dunal. Estimation of the total flavonoids in the plant extracts was carried out. Antioxidant and antilipid peroxidation activities of. Assessment of antioxidant activity of plant extracts by. Screening of antioxidant activity and phenolic content of 24. This result identifies this plant as a good candidate for further investigations. The concentration of extracts in lard varied from 0.
Antioxidant activity of plant extracts and their effect on. Antioxidant activity of various tea extracts in relation to. Tocopherol was used as a standard to evaluate the antioxidative activity of samples. Determination of antioxidant activity the antioxidant activity of plant extracts against peroxidation of linoleic acid was determined by the reported method 16. Antioxidant properties of extracts from selected plant materials. The aim of this study was to screen various solvent extracts of whole plant of torilis leptophylla to display potent antioxidant activity in vitro and in vivo, total phenolic and flavonoid contents in order to find possible sources for future novel antioxidants in food and pharmaceutical formulations. The antioxidant effects were evaluated using in vitro, 2, 2diphenylpicrylhydrazine photometric assay and in vivo serum catalase, superoxide dismutase.
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